FastDigest PaeI

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	The murine miR302pEGFP fragment was cut from the “pmmiR302pEGFP” vector (Rahimi et al., 2018b) with AseI and PaeI. This fragment included the murine miR-302 core promoter region from −595 to +45 (chr3:127,544,494-127,545,132) and the egfp CDS. The pUC19 (GenBank accession No. M77789.2) vector digested with NdeI and PaeI was used as a backbone to ligate the miR302pEGFP fragment in.

Protocol tips
The murine miR302pEGFP fragment was cut from the “pmmiR302pEGFP” vector (Rahimi et al., 2018b) with AseI and PaeI. This fragment included the murine miR-302 core promoter region from −595 to +45 (chr3:127,544,494-127,545,132) and the egfp CDS. The pUC19 (GenBank accession No. M77789.2) vector digested with NdeI and PaeI was used as a backbone to ligate the miR302pEGFP fragment in.

Publication protocol

The murine miR302pEGFP fragment was cut from the “pmmiR302pEGFP” vector (Rahimi et al., 2018b) with AseI and PaeI. This fragment included the murine miR-302 core promoter region from −595 to +45 (chr3:127,544,494-127,545,132) and the egfp CDS. The pUC19 (GenBank accession No. M77789.2) vector digested with NdeI and PaeI was used as a backbone to ligate the miR302pEGFP fragment in. The resulting vector “pUC19-pmmiR302pEGFP” was digested with XhoI and Bsp119I. The sequence coding for EGFP-NEO separated by the self splicing T2A peptide was obtained from the “pUC57-pPB.TET.GFP.RFP” vector (kindly provided by Dr. Mark Denham, Aarhus University) digested with XhoI and Bsp119I. The egfp-neo fusion sequence was ligated into the linearized “pUC19-pmmiR302pEGFP” to give the “pmmiR302pEGFP-NEO” vector (Fig. S1A). This vector was linearized with ApaLI for ES cell transfection.

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