BstZ17I-HF®

Restriction Enzymes BstZ17I / Bst1107I

Experiment

Restriction Enzymes BstZ17I / Bst1107I

Product

BstZ17I-HF® from New England BioLabs

Manufacturer

New England BioLabs

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Protocol tips

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	The annealed DNA icosahedron samples were treated with different restriction enzyme cocktails for corresponding patterns (BsiWI-HF, BstZ17I-HF, MfeI-HF, MluI-HF for pattern I; AclI, BamHI-HF, MfeI-HF, MluI-HF for pattern II; AclI, BamHI-HF, BsiWI-HF, BstZ17I-HF, MfeI-HF, MluI-HF for pattern III). One unit of each enzyme was used to digest the icosahedron in 50 µL 1 × CutSmart buffer at 37 °C for 2 h. Additional details can be found in Supplementary Methods.

Protocol tips
The annealed DNA icosahedron samples were treated with different restriction enzyme cocktails for corresponding patterns (BsiWI-HF, BstZ17I-HF, MfeI-HF, MluI-HF for pattern I; AclI, BamHI-HF, MfeI-HF, MluI-HF for pattern II; AclI, BamHI-HF, BsiWI-HF, BstZ17I-HF, MfeI-HF, MluI-HF for pattern III). One unit of each enzyme was used to digest the icosahedron in 50 µL 1 × CutSmart buffer at 37 °C for 2 h. Additional details can be found in Supplementary Methods.

Protocol tips

The annealed DNA icosahedron samples were treated with different restriction enzyme cocktails for corresponding patterns (BsiWI-HF, BstZ17I-HF, MfeI-HF, MluI-HF for pattern I; AclI, BamHI-HF, MfeI-HF, MluI-HF for pattern II; AclI, BamHI-HF, BsiWI-HF, BstZ17I-HF, MfeI-HF, MluI-HF for pattern III). One unit of each enzyme was used to digest the icosahedron in 50 µL 1 × CutSmart buffer at 37 °C for 2 h. Additional details can be found in Supplementary Methods.

Publication protocol

"Restriction enzyme cleavage
The annealed DNA icosahedron samples were treated with different restriction enzyme cocktails for corresponding patterns (BsiWI-HF, BstZ17I-HF, MfeI-HF, MluI-HF for pattern I; AclI, BamHI-HF, MfeI-HF, MluI-HF for pattern II; AclI, BamHI-HF, BsiWI-HF, BstZ17I-HF, MfeI-HF, MluI-HF for pattern III). One unit of each enzyme was used to digest the icosahedron in 50 µL 1 × CutSmart buffer at 37 °C for 2 h. Additional details can be found in Supplementary Methods."

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Papers

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Manufacturer protocol

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