SpeI-HF®

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	‘Digestion Premix’ containing 1 μl of 10× RE solution, 3 μl of 2 M trehalose, 5.5 μl of DW, and 0.5 μl of restriction endonuclease, SpeI-HF, was added to the beads, and suspended by pipetting up and down. The suspension was incubated at 37 °C for 15 min, followed by mixing by pipetting up and down and further incubation at 37 °C for 15 min. Then, the beads were collected by magnet to remove the supernatant. The beads were rinsed twice, as described in the ‘Beads-rinsing’ section above.

Protocol tips

‘Digestion Premix’ containing 1 μl of 10× RE solution, 3 μl of 2 M trehalose, 5.5 μl of DW, and 0.5 μl of restriction endonuclease, SpeI-HF, was added to the beads, and suspended by pipetting up and down. The suspension was incubated at 37 °C for 15 min, followed by mixing by pipetting up and down and further incubation at 37 °C for 15 min. Then, the beads were collected by magnet to remove the supernatant. The beads were rinsed twice, as described in the ‘Beads-rinsing’ section above.

Publication protocol

"Removal of 200-bp downstream DNA fragment
‘Digestion Premix’ containing 1 μl of 10× RE solution, 3 μl of 2 M trehalose, 5.5 μl of DW, and 0.5 μl of restriction endonuclease, SpeI-HF, was added to the beads, and suspended by pipetting up and down. The suspension was incubated at 37 °C for 15 min, followed by mixing by pipetting up and down and further incubation at 37 °C for 15 min. Then, the beads were collected by magnet to remove the supernatant. The beads were rinsed twice, as described in the ‘Beads-rinsing’ section above."

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Manufacturer protocol

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