SpeI restriction enzyme

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Their stock concentrations were 15 U/µl for EcoRV (Cat # : D1042A), 15 U/µl for PstI (Cat # : D1073A), 10 U/µl for XhoI (Cat # : D1094A), 15 U/µl for XbaI (Cat # : D1093A), 10 U/µl for SpeI (Cat # : D1086A), and 10 U/µl for SphI (Cat # : D1180A), respectively. EcoRV creates a blunt end, XbaI, SpeI and XhoI generate a 5′-overhang, and SphI and PstI create a 3′-overhang.

Protocol tips
Their stock concentrations were 15 U/µl for EcoRV (Cat # : D1042A), 15 U/µl for PstI (Cat # : D1073A), 10 U/µl for XhoI (Cat # : D1094A), 15 U/µl for XbaI (Cat # : D1093A), 10 U/µl for SpeI (Cat # : D1086A), and 10 U/µl for SphI (Cat # : D1180A), respectively. EcoRV creates a blunt end, XbaI, SpeI and XhoI generate a 5′-overhang, and SphI and PstI create a 3′-overhang.

Publication protocol

Nucleotide analogue 2′-OMeN or PS was used to substitute the recognition sequences of restriction endonucleases. These oligonucleotides at HPLC grade were purchased from Sangon Biotech Co. Ltd. (Shanghai, China), and were prepared at the concentration of 100 µM in ddH2O without further purification. These stock solutions were stored in −20°C. Six restriction endonucleases were purchased from TaKaRa Biotechnology Company (Dalian, China). Their stock concentrations were 15 U/µl for EcoRV (Cat # : D1042A), 15 U/µl for PstI (Cat # : D1073A), 10 U/µl for XhoI (Cat # : D1094A), 15 U/µl for XbaI (Cat # : D1093A), 10 U/µl for SpeI (Cat # : D1086A), and 10 U/µl for SphI (Cat # : D1180A), respectively. EcoRV creates a blunt end, XbaI, SpeI and XhoI generate a 5′-overhang, and SphI and PstI create a 3′-overhang.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Restriction Enzymes SpeI / BcuI using SpeI restriction enzyme from Takara Bio Inc.

Manufacturer protocol

Download the product protocol from Takara Bio Inc for SpeI restriction enzyme below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Restriction Enzymes SpeI / BcuI using SpeI restriction enzyme from Takara Bio Inc. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.