FastDigest SsiI

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	NA digestion reactions were set up with 200 ng DNA and 1 µL of each enzyme (AciI, MspI or HpaII FastDigest - purchased from Fermentas) in a total volume of 20 µL and incubated for 1 h at 37°C. Reaction products were loaded on 2% MetaPhor high resolution agarose (Lonza) gel with 0.4 µg/mL of Ethidium Bromide.

Protocol tips
NA digestion reactions were set up with 200 ng DNA and 1 µL of each enzyme (AciI, MspI or HpaII FastDigest - purchased from Fermentas) in a total volume of 20 µL and incubated for 1 h at 37°C. Reaction products were loaded on 2% MetaPhor high resolution agarose (Lonza) gel with 0.4 µg/mL of Ethidium Bromide.

Publication protocol

Testing controls (unmethylated and methylated DNA fragments) by methylation sensitive restriction enzyme assays. DNA digestion reactions were set up with 200 ng DNA and 1 μL of each enzyme (AciI, MspI or HpaII FastDigest— purchased from Fermentas) in a total volume of 20 μL and incubated for 1 h at 37°C. Reaction products were loaded on 2% MetaPhor high resolution agarose (Lonza) gel with 0.4 μg/mL of Ethidium Bromide.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for FastDigest SsiI below.

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