ScaI restriction enzyme

Protocol tips

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	Approximately 500 ng of amplified DNA was digested with ScaI (TAKARA, Japan) at 37℃ overnight.

Protocol tips
Approximately 500 ng of amplified DNA was digested with ScaI (TAKARA, Japan) at 37℃ overnight.

Publication protocol

Afterwards, approximately 500 ng of amplified DNA was digested with ScaI (TAKARA, Japan) at 37℃ overnight. In case there was a G allele, the amplicon was cut into the 576 bp, 174 bp and 147 bp fragments. In case there was a T allele, we obtained the 750 bp and 147 bp fragments after digestion. The resulting products were analyzed by electrophoresis on a 1.2% agarose gel or 8% polyacrylamide gel (PAGE) following ethidium bromide staining (Fig 1).

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Manufacturer protocol

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