ScaI R6211

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Each 10 μL of DRB1 PCR product was digested with 5 U of SacI, Hin1I, HaeIII, MvaI, and SacII, respectively, in a total volume of 20 μL, including 2 μL 10× buffer. Each 10 μL of DQB1 PCR product was digested with 5 U of MroxI, ScaI, SacII, NciI, TaqI, MvaI, and HaeIII, respectively.

Protocol tips
Each 10 μL of DRB1 PCR product was digested with 5 U of SacI, Hin1I, HaeIII, MvaI, and SacII, respectively, in a total volume of 20 μL, including 2 μL 10× buffer. Each 10 μL of DQB1 PCR product was digested with 5 U of MroxI, ScaI, SacII, NciI, TaqI, MvaI, and HaeIII, respectively.

Publication protocol

The cleavage map typing method and allele nomenclature referred to that of Konnai et al. [20]. Each 10 μL of DRB1 PCR product was digested with 5 U of SacI, Hin1I, HaeIII, MvaI, and SacII, respectively, in a total volume of 20 μL, including 2 μL 10× buffer. Each 10 μL of DQB1 PCR product was digested with 5 U of MroxI, ScaI, SacII, NciI, TaqI, MvaI, and HaeIII, respectively. Samples were resolved by agarose gel electrophoresis at varying concentrations (Table S1) (see Supplementary Material available online at http://dx.doi.org/10.1155/2014/272601).

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