Cell Counting Kit-8

Protocol tips

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	- Plate was incubated at 37ºC for 4 hours and then quantifiably measured at a wavelength of 570 nm.

Protocol tips
- Plate was incubated at 37ºC for 4 hours and then quantifiably measured at a wavelength of 570 nm.

Publication protocol

MDA-MB-231 cells were plated at density of 1,000 cells/well in a 96-well plate. Then, the indicated concentration of DPPA was added 24 hours later, and each concentration was tested in quadruplicate. Cell viability and cell proliferation were evaluated using the Cell Counting Kit-8 (CCK8) assay. The CCK8 reagent was added at the indicated time, and the plate was incubated at 37ºC for 4 hours and then quantifiably measured at a wavelength of 570 nm. The data were obtained from three independent experiments. A colony formation assay was also used to detect cell proliferation. Briefly, MDA-MB-231 cells (3,000 cells/well) and 4T1 cells (1,500 cells/well) were seeded into 6-well plates. Then, the indicated concentrations of DMSO and DPPA were added into the wells 24 hours later. The colonies were fixed and stained after 7 days of growth. The effect of each treatment was assessed from three replicates and three independent experiments.

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Manufacturer protocol

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