Bsp120I (PspOMI) (10 U/µL)

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	Isolates of L. garvieae were molecularly characterized by PFGE using the restriction enzymes Bsp120I (MBI Fermentas) as described by Vela et al. (2001). The fragments were resolved by PFGE in electrophoresis‐grade agarose (1%; Boehringer Mannheim, Mannheim, Germany) with a CHEF‐DR III System (Bio‐Rad, Alcobendas, Madrid, Spain).

Protocol tips
Isolates of L. garvieae were molecularly characterized by PFGE using the restriction enzymes Bsp120I (MBI Fermentas) as described by Vela et al. (2001). The fragments were resolved by PFGE in electrophoresis‐grade agarose (1%; Boehringer Mannheim, Mannheim, Germany) with a CHEF‐DR III System (Bio‐Rad, Alcobendas, Madrid, Spain).

Publication protocol

Isolates of L. garvieae were molecularly characterized by PFGE using the restriction enzymes Bsp120I (MBI Fermentas) as described by Vela et al. (2001). The fragments were resolved by PFGE in electrophoresis‐grade agarose (1%; Boehringer Mannheim, Mannheim, Germany) with a CHEF‐DR III System (Bio‐Rad, Alcobendas, Madrid, Spain). XbaI‐digested DNA from Salmonella enterica serotype Braenderup H9812 and lambda ladder PFGE marker (Boehringer Mannheim) were used for the molecular weight size determination. PFGE patterns were examined visually, and isolates were considered unique when they differed in at least one band. Similarities between restriction endonuclease digestion profiles of the different isolates were expressed using the Dice similarity index, with the numerical taxonomy program BioNumerics (Applied Maths BVBA, Sint‐Martens‐Latem, Belgium). A similarity matrix was computed and transformed into an agglomerative cluster using the unweighted pair group method with arithmetic averages (UPGMA). Genetic diversity (GD) was calculated as the ratio between the total number of PFGE patterns and the total number of isolates (Martínez et al. 2002).

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