FspBI (BfaI) (10 U/µL)

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	The restriction enzymes RsaI and FspBI (Fermentas, York, UK) discriminated the respective genotypes.

Protocol tips
The restriction enzymes RsaI and FspBI (Fermentas, York, UK) discriminated the respective genotypes.

Publication protocol

Genotyping for rs166850 and rs10451941 was performed by restriction fragment-length polymorphism analysis with specific primers for both genotypes (sense, 5′-CCC TTT TAG TTT TTA CGA TGA AGA-3′; antisense, 5′-TTG CTT AAG ACA TTA CTT GGA ACA-3′). The restriction enzymes RsaI and FspBI (Fermentas, York, UK) discriminated the respective genotypes.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for FspBI (BfaI) (10 U/µL) below.

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