Protocol tips
| Upstream tips |
Protocol tips |
Downstream tips |
|
Enzyme digestion was conducted in a 20 μL final volume with 5U of BstUI enzyme (New England Biolabs [NEB]) and 10 μL of PCR product. The reaction was incubated at 60℃ overnight. The digested products were visualized on 3% agarose gels stained with ethidium bromide. |
|
| Protocol tips |
| Enzyme digestion was conducted in a 20 μL final volume with 5U of BstUI enzyme (New England Biolabs [NEB]) and 10 μL of PCR product. The reaction was incubated at 60℃ overnight. The digested products were visualized on 3% agarose gels stained with ethidium bromide. |
Publication protocol
Enzyme digestion was conducted in a 20 μL final volume with 5U of BstUI enzyme (New England Biolabs [NEB]) and 10 μL of PCR product. The reaction was incubated at 60℃ overnight. The digested products were visualized on 3% agarose gels stained with ethidium bromide.
Full paper
Login or
join for free to view the full paper.
Reviews
BstUI NEB#R0518 from New England BioLabs has not yet been reviewed for this experiment
We'd love it if you would be the first to write a review!
Discussion
Start your discussion
Share your thoughts or question with experts in your field
Start a discussion
Papers
Check out relevant papers found by Labettor's AI that are relevant for performing Restriction Enzymes Bsh1236I / BstUI using BstUI NEB#R0518 from New England BioLabs.
Manufacturer protocol
Download the product protocol from New England BioLabs for BstUI NEB#R0518 below.
We haven't found the manufacturer protocol for this product yet.
Videos
Check out videos that might be relevant for performing Restriction Enzymes Bsh1236I / BstUI using BstUI NEB#R0518 from New England BioLabs. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.
We haven't found any additional videos for this experiment / product combination yet.