Lysozyme/Muramidase

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IHC and AB staining were performed as we have described previously.62 Tissue was processed for routine IHC staining using the following antibodies: rabbit anti-LYZ (Diagnostic BioSystems, Pleasanton, CA), anti-cyclin D1 (Abcam, Cambridge, MA), anti-Olfm4 (Cell Signaling, Danvers, MA), and anti-Ki67 (Novus Biologicals, Centennial, CO). Negative controls (including no primary antibody or isotype-matched mouse immunoglobulin G) were used in each assessment. AB staining was performed according to standard protocol using AB pH 2.5 Stain Kit (Dako, Carpinteria, CA). IAP staining was performed using Vulcan Fast Red Chromogen kit (Biocare Medical, Concord, CA), following the manufacturer’s recommendations. Formalin-fixed, paraffin-embedded tissue samples of normal human small bowel and colon were utilized for SIRT2 staining using anti-SIRT2 antibody (Novus Biologicals, Centennial, CO).

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