Recombinant Anti-Lysozyme antibody [EPR2994(2)] (ab108508)

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Publication protocol

Mice were killed at 3.5 days WBI, and the small intestine was fixed with neutral formalin. Then the tissues were dehydrated and embedded with paraffin. Then the sections were boiled in 10 mM/L citrate buffer solution (pH 9.0) for antigen retrieval according to the standard procedures. After antigen retrieval, the sections were incubated with serum for 1 h at room temperature to block non-specific antigen-binding sites, and then with anti-Lgr5 antibody (1:50 dilution, Abcam, Cambridge, MA, USA), anti-Ki67 antibody (1:300 dilution, Novus a biotechne brand), anti-lysozyme (1:800 dilution, Abcam, Cambridge, MA, USA) or anti-villi (1:800 dilution, Abcam, Cambridge, MA, USA) overnight at 4 °C. Sections were then incubated in secondary antibody for 30 min at 37 °C. Positive cells were detected using DAB kit (Sigma Aldrich). The images were captured and positive staining was quantified objectively by the IPP software as described previously in a blinded fashion.

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