β-catenin Antibody (E-5): sc-7963

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Publication protocol

Tissues fixed in 10% formalin were embedded in paraffin, and 4-μm sections cut onto Superfrost Plus glass slides (Thermo Fisher Scientific, Pittsburgh, PA) were used for H&E, Sirius Red, or immunohistochemical analysis as described elsewhere1. Primary antibodies used include -catenin (1:100, sc-7199), glutamine synthetase (GS; 1:50, sc-9067), cyclin D1 (1:50, sc-753), and CD45 (1:100, sc-53665) from Santa Cruz Biotechnology, Dallas, TX; Ki67 (1:100, RM9106-S) from Thermo Fisher Scientific; -SMA (1:200, M0851) from Agilent, Santa Clara, CA; CD68 (1:100, MCA1957) and F4/80 (1:100, MCA497GA) from Bio-Rad, Hercules, CA; Neutrophil Elastase (1:1500, ab68672) from Abcam, Cambridge, MA; Sox9 (1:100, AB5535) from Millipore, Temecula, CA; and EpCAM (1:10, Clone G8.8) from Developmental Studies Hybridoma Bank, University of Iowa. Apoptosis was determined using terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining (ApopTag Peroxidase in Situ Apoptosis Detection Kit, Millipore).

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