Cell Counting Kit-8

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Pre-incubate the plate for 24 hours in a humidified incubator. Be careful not to introduce bubbles to the wells, since they interfere with the O.D. reading.		- After cells were washed they were irradiated with 5 J/cm2 light dose using a 635 nm laser at a power density of 20 mW/cm2.

Upstream tips
- Pre-incubate the plate for 24 hours in a humidified incubator. Be careful not to introduce bubbles to the wells, since they interfere with the O.D. reading.
Downstream tips
- After cells were washed they were irradiated with 5 J/cm2 light dose using a 635 nm laser at a power density of 20 mW/cm2.

Publication protocol

Cell cytotoxicity was evaluated by determining the viability of HeLa cells with a colorimetric tetrazolium salt-based assay, CCK8. HeLa cells were seeded in 96-well culture plates at 1 × 104 cells per well. After being cultured for 24 h, the cell culture medium was removed and the DMEM containing the functional SWCNTs was added into each well, followed by incubation at 37 °C for 2 h. The cells were washed to remove the unbound functional SWCNTs and irradiated with 5 J/cm2 light dose using a 635 nm laser at a power density of 20 mW/cm2. Cell cytotoxicity was assessed 24 h after the laser irradiation with CCK8. The absorbance of each well at 450 nm was measured by Infinite M200 (TECAN, Mannedorf, Switzerland) to determine the cell viability

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