Anti-Glucagon antibody [K79bB10] (ab10988)

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Publication protocol

C57BL/6J mice fed HFHSD (22-week old males) were anesthetized and perfused transcardially with ice-cold, 0.05 M phosphate-buffered saline (pH 7.4), followed by 4% paraformaldehyde (PFA) for fixation. The pancreas and kidney were dissected and fixed in 4% PFA at 4 °C. Samples were frozen in OCT compound and sectioned in 5-μm thick slices. Human tissue sections were purchased (BioChain Institute Inc., USA). For immunostaining, we used rabbit polyclonal anti-SGLT1 (ab14685; abcam, Japan), rabbit polyclonal anti-SGLT2 (ab85626; abcam, Japan), guinea pig polyclonal anti-insulin (A0564; Dako, Japan), and mouse monoclonal anti-glucagon (ab10988; abcam, Japan) antibodies. We visualized immune complexes with FITC- or CY3-conjugated secondary antibodies.

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