Anti-Glucagon Antibody, clone 13D11.33

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Publication protocol

Tissues were fixed by transcardiac perfusion of 4% paraformaldehyde in PBS for 10–15 min, followed by 6 h immersion in the same fixative. Overnight fixation was avoided to preserve fluorescence of dtTomato protein. Cryosections (6 μm) were used for all histological analyses. The primary antibodies used are listed in Table 1. These antibodies were validated using mouse tissues known to express and not to express the target protein. Secondary antibodies were obtained from Jackson ImmunoResearch (West Grove, PA, USA). For staining with Cre antibody, an antigen retrieve was performed using a citrate-microwave method according to the manufacture’s protocol.

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