Anti-Insulin antibody (ab7842)

Protocol tips

Upstream tips	Protocol tips	Downstream tips

Publication protocol

5μm sections of paraffin-embedded fixed mouse pancreas were boiled in 0.01M citric acid buffer (pH 6.0) for 2.5 min for antigen retrieval, blocked with 1% BSA, 10% normal goat serum, 0.1% Triton X-100 in PBS for 1 hour then incubated at 4°C overnight with rabbit anti-CXCL14 antibody (1:500), guinea pig anti-insulin (1:200), mouse anti-glucagon (1:50) and rat anti-somatostatin (1:25) antibodies. Sections were exposed for 1 hour at room temperature to Alexa Fluor® 488 anti-rabbit secondary antibody (1:150) and species-specific Alexa Fluor® 594 secondary antibodies (all at 1:200). Nuclei were detected using DAPI (1:500). Immunostained pancreas sections were analysed using Image J software and the proportion of islet cells expressing CXCL14 was calculated by dividing the mean number of CXCL14-positive cells by the number of β, a- or δ-cells per islet.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Immunohistochemistry Mouse - Insulin using Anti-Insulin antibody (ab7842) from Abcam.

Manufacturer protocol

Download the product protocol from Abcam for Anti-Insulin antibody (ab7842) below.

Videos

Check out videos that might be relevant for performing Immunohistochemistry Mouse - Insulin using Anti-Insulin antibody (ab7842) from Abcam. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.