Recombinant Anti-VEGFA antibody [EP1176Y] - C-terminal (ab52917)

Protocol tips

Upstream tips	Protocol tips	Downstream tips

Publication protocol

Primary anti-VEGFA (ab52917, Abcam, USA), anti-vWF (PB0273, Boster, Wuhan, China), anti-PCNA (BM0104, Boster, Wuhan, China), anti-LC3 (WL01506, Wanlei, Shenyang, China), anti-p62 (WL02385, Wanlei, Shenyang, China), anti-ERα (ab32063, Abcam, USA), and anti-PR (sc-810, Santa Cruz, TX, USA) antibodies were diluted in 0.5% goat serum in PBS. The sections were heated in a microwave in sodium citrate solution for antigen recovery and pretreated with 0.3% H2O2 in methanol to quench endogenous peroxidase activity. Then, the samples were incubated with 3% goat serum to block nonspecific antibody binding sites. Subsequently, the samples were incubated with primary antibodies at 4 °C overnight. Immunoreactivity was visualized using a Mouse and Rabbit Specific HRP/DAB Detection IHC kit (ab64264, Abcam) according to the manufacturer’s instructions.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Immunohistochemistry Mouse - VEGFA using Recombinant Anti-VEGFA antibody [EP1176Y] - C-terminal (ab52917) from Abcam.

Manufacturer protocol

Download the product protocol from Abcam for Recombinant Anti-VEGFA antibody [EP1176Y] - C-terminal (ab52917) below.

Videos

Check out videos that might be relevant for performing Immunohistochemistry Mouse - VEGFA using Recombinant Anti-VEGFA antibody [EP1176Y] - C-terminal (ab52917) from Abcam. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.