LC3B Antibody

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Frozen uterine sections (10 μm) were used for immunohistochemistry and immunofluorescence as previously described [11, 21] using the following antibodies: anti-PCNA (Proliferating Cell Nuclear Antigen) antibody (1:1000, D3H8P, Cell Signaling Technology), anti-E-cadherin antibody (1:300, 24E10, Cell Signaling Technology), and anti-LC3/Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3A) antibody (1:300, NB100–2220, Novus Biologicals). Quantification of LC3 staining in D18.5 and postpartum day 1 (PPD1) uterine luminal epithelium (LE) was done as follow: LC3 staining in at least three representative LE areas and nearby myometrium areas (same size) from the same section was quantified using ImageJ [11, 12, 22]. The ratio of average LE staining intensity/average myometrium staining from one section per mouse was counted as one data point. N = 3 mice per genotype per time point.

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