Anti-Estrogen Receptor α Antibody

Immunohistochemistry Mouse - ERα

Experiment
Immunohistochemistry Mouse - ERα
Product
Anti-Estrogen Receptor α Antibody from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Publication protocol

Sections were washed three times in PB 0.1M for 5 min each and then microwaved in 10 mM citrate buffer pH 6.0 (4 min at 800 W followed by 2 × 5 min at 400 W) for antigen unmasking. Sections were then washed in PB 0.1M, and incubated in blocking solution (5% donkey serum + 0.3% Triton X-100) in PB 0.1M for 60 min. Then, sections were incubated in rabbit anti-ERα (1/500; Millipore, Cat# 06–935 Lot#2708536, RRID:AB_310305) or guinea pig anti-GnRH (1/3000 raised by Dr. Erik Hrabovszky, Laboratory of Endocrine Neurobiology, Institute of Experimental Medicine of the Hungarian Academy of Sciences, Budapest, Hungary) and sheep anti-nNOS (1/10,000; generous gift from Dr. P. C. Emson, Medical Research Council, Laboratory for Molecular Biology, Cambridge, UK) and, when required, chicken anti-GFP antibody (1/500; Abcam, Cat# ab13970 Lot#GR236651–14 RRID:AB_300798) in blocking solution for 48 hr at 4°C. After incubation in the primary antibody, sections were rinsed with PB 0.1M three times for 10 min each and then incubated in the secondary antibody: Alexa-568-conjugated donkey anti-rabbit or anti-guinea pig (Thermo Fisher Scientific Cat# A10042, RRID:AB_2534017), Alexa-647-conjugated donkey anti-sheep (Thermo Fisher Scientific Cat# A-21448, RRID:AB_2535865) and donkey anti-chicken Alexa 488 (Jackson ImmunoResearch Labs Cat# 703–545-155, RRID:AB_2340375) for 60 min at room temperature. Sections were then washed, counterstained with Hoechst (1/10,000; Thermo Fisher Scientific Cat# H3569, RRID: AB_2651133) for 5 min, washed twice for 5 min and coverslipped with Mowiol coverslip mounting solution.

Full paper   Login or join for free to view the full paper.

Reviews

Anti-Estrogen Receptor α Antibody from Sigma-Aldrich has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Immunohistochemistry Mouse - ERα using Anti-Estrogen Receptor α Antibody from Sigma-Aldrich.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Anti-Estrogen Receptor α Antibody below.

We haven't found the manufacturer protocol for this product yet.

Videos

Check out videos that might be relevant for performing Immunohistochemistry Mouse - ERα using Anti-Estrogen Receptor α Antibody from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms