Estrogen Receptor alpha Antibody (F-10): sc-8002

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To identify cells expressing ERα, FOXA1, and GATA-3, formalin-fixed mammary gland sections were immunostained using antibodies against ERα, FOXA1, and GATA-3 (Santa Cruz Biotechnology, Santa Cruz, CA) as described previously (2). Expression was recorded in two parts; percentage of staining that ranged from ‘0’ in case of no nuclear expression to a maximum of ‘100’ with all cells showing expression; and intensity of 0, 1+, 2+ and 3+ for none, weak, moderate and strong staining respectively. Percentage (P) and intensity (I) of nuclear expression for ERα, FOXA1 and GATA3 were multiplied to generate a numerical expression score (S = P × I) (2).

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