CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS)

Protocol tips

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-cells were plated in 96-well plates in medium containing 10% FBS at a density of ~3,000 cells/well.

Upstream tips
-cells were plated in 96-well plates in medium containing 10% FBS at a density of ~3,000 cells/well.

Publication protocol

For the quantification of the cell viability, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxy-methoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) assays were performed according to the manufacturer’s instructions (CellTiter 96 AQueous Non-Radioactive Cell Proliferation assay; Promega, Madison, WI, USA). Briefly, the cells were plated in 96-well plates in medium containing 10% FBS at a density of ~3,000 cells/well. The absorbance was measured at 490 nm using a microtitration plate spectrophotometer.

For the colony formation assays, dissociated cells were planted into 6-cm cell culture dishes (100 cells/dish) and incubated for 12 days. The plates were washed with PBS and stained with Giemsa. The number of colonies consisting of >50 cells was counted.

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Manufacturer protocol

Download the product protocol from Promega for CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) below.

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