Mouse Osteopontin/OPN Antibody

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In FB-labelled TL and LS DRG sections from perfused-fixed mice, specific antibodies against TrkC, Gfrα2, Gfrα3, Nrp1 and Spp1 were used to determine the relative expression of these proteins in colonic sensory neurons and further validate clusters derived from scRNAseq data. No labelling was observed where primary antibodies were excluded. Species-specific secondary antibodies conjugated to fluorophores (AlexaFluor-488/AlexaFluor-568) were used to visualise labelling. Relative intensity of immunostaining reaction products were determined for all colon-labelled DRG neurons with visible nuclei from 2 DRG per spinal segment from two to three animals.

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