Mucin 2 Antibody (Ccp58): sc-7314

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Tissue sections were deparaphinized, hydrated and washed in pH 7.2 PBS. Endogenous peroxidase activity was eliminated by treatment with H2O2 40vv solution at 0.2% PBS and unspecific binding was blocked by incubation with goat serum diluted at 1:40. Next, sections were incubated with monoclonal anti-MUC-2 antibody (Santa Cruz Biotechnology, Santa Cruz, USA) diluted at 1:150 for 18 hours, then incubated with biotin-labeled IgG at 1:100 (Bethyl Laboratories Inc, Montgomery, USA) and peroxidase-conjugated streptavidin at 1:100 for 1 hour (Zymed Laboratories Inc., San Francisco, USA). Color reaction was developed using DAB 0.05% in H2O2 40vv at 0.2%. Histological sections of caeca obtained from CTRL-WT mice were used as positive control. As negative control, PBS-treated sections were used. All sections were stained with Harris haematoxylin, hydrated and diaphanized for entelan assembly.

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