CD133 (Prominin-1) Monoclonal Antibody (13A4), APC, eBioscience™

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Publication protocol

Paraffinated hepatic slices were de-paraffinization with xylene and rehydrated with a gradual concentration of ethanol. Tissues were incubated with the appropriate primary antibody for overnight at 4°C based on the manufacturers’ protocol, followed by incubation with the secondary anti-rat-biotinylated antibody (Vectastain Elite ABC Kit). The complex streptavidin-biotin was visualized with the DBA peroxidase substrate kit (Vector Laboratories, UK). Slides were observed under a Leica DM2000 microscope (Leica Camera AG, Solms, Germany). Primary antibodies used were anti-CD34 (clone MEC 14.7, Santa Cruz Biotechnology, Dallas, USA) and anti-CD133 (clone 13A4, eBioscience, Milan, Italy).

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