Cell Proliferation ELISA, BrdU

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- 3,000 cells/well were seeded in a 96 well plate	- Cells were labelled with BrdU for 4 h.

Upstream tips
- 3,000 cells/well were seeded in a 96 well plate
Protocol tips
- Cells were labelled with BrdU for 4 h.

Publication protocol

For the cell proliferation assay, a cell proliferation ELISA kit for the thymidine analog 5-bromo-2′-deoxyuridine (BrdU; Roche Diagnostics, Mannheim, Germany) was used as per the manufacturer’s instructions. In brief, cells were seeded in 96-well plates and the initial cell number was adjusted to 3,000/well. Following drug treatment, the cells were labeled with BrdU for 4 h. Subsequently, anti-BrdU-POD Fab fragments and substrate were added to the medium. The optical density (OD) was determined at 405 nm using an iMark Microplate Reader. The results were normalized to the control (the group treated with vehicle).

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