Anti-SOX9 Antibody AB5535

Protocol tips

Upstream tips	Protocol tips	Downstream tips

Publication protocol

To identify the type of Evc2-expressing cells, fluorescence IHC was performed with anti-β-gal and anti-SOX9 (EMD Millipore) antibodies using Het and KO embryos at E16.5 with DAPI nuclear staining as previously described (20). For staining with WT embryos at E16.5, anti-EVC2 (sc-28393, Santa Cruz Biotechnology, Inc) and anti-SOX9 antibodies were used. Alexa Fluor 488 (for anti-SOX9) and 594 (for anti-β-gal or anti-EVC2) (both Life Technologies) were used as secondary antibodies and mounted with ProLong Gold antifaint mountant with DAPI (Life Technologies)

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Immunohistochemistry Mouse - SOX9 using Anti-SOX9 Antibody AB5535 from Merck Millipore.

Manufacturer protocol

Download the product protocol from Merck Millipore for Anti-SOX9 Antibody AB5535 below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Immunohistochemistry Mouse - SOX9 using Anti-SOX9 Antibody AB5535 from Merck Millipore. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.