Anti-Sox9 Antibody AB5535

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Publication protocol

266-6 cells were fixed 20 min at −20°C in methanol, washed three times in PBS, and permeabilised in PBS, 0.3% Triton X-100 for 10 min and incubated in blocking buffer (PBS, 10% BSA, 3% milk powder, 0.3% Triton X-100) for 1 h. Mouse tissue samples were fixed overnight in 4% paraformaldehyde in PBS at 4° C, and embedded in paraffin. Archival human formalin-fixed pancreatic samples from the Cliniques Universitaires Saint-Luc Pathology Bank were used to perform immunolabelling. Serial paraffin sections, 9 μm thick, were mounted on glass slides, then deparaffinised and re-hydrated. Antigen retrieval was performed by boiling the slides for 10 min in 10 mM citrate buffer, pH 6, in a microwave oven.

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