Anti-Collagen X antibody (ab58632)

Protocol tips

Upstream tips	Protocol tips	Downstream tips

Publication protocol

Standard protocol of immunohistochemistry were perform in current study. Sagittal sections of knee joint medial compartment were incubated with primary antibodies against Matrix Metallo Preteinases 13 (MMP-13) (Abcam, 1:100, ab39012), Collagen X (Abcam, 1:100, ab58632), Collagen II (Abcam, 1:400, ab ab34712), phosphorylated Smad2/3 (pSmad2/3) (Santa Cruz Biotechnology Inc., 1:40),TGF-β1(Abcam, 1:50, ab92486), Vascular Endothelial Growth Factor Receptor-2 (VEGFR2) (Abcam, 1:100, ab2349) and osterix (Abcam, 1:500, 22552) overnight at 4 °C. After used a horseradish peroxidase–streptavidin detection system (ZSGB BIO) to detect the immunoactivity, counterstaining was performed with hematoxylin (ZSGB BIO). Tartrate-resistent acid phosphatase (TRAP) staining was performed following a standard protocol (Sigma-Aldrich).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Immunohistochemistry Mouse - Col X using Anti-Collagen X antibody (ab58632) from Abcam.

Manufacturer protocol

Download the product protocol from Abcam for Anti-Collagen X antibody (ab58632) below.

Videos

Check out videos that might be relevant for performing Immunohistochemistry Mouse - Col X using Anti-Collagen X antibody (ab58632) from Abcam. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.