CytoTox 96® Non-Radioactive Cytotoxicity Assay

Protocol tips

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- 3,000 cells/well were seeded for the assay.

Upstream tips
- 3,000 cells/well were seeded for the assay.

Publication protocol

Cell death was evaluated using a lactate dehydro genase (LDH) release assay. LDH release was quantified with a CytoTox 96 non-radioactive cytotoxicity assay kit (Promega, Madison, WI, USA) according to the manufacturer’s instructions. Cells were seeded in 96-well plates and the initial cell number was adjusted to 3,000/well. Following drug treatment, 50 μl medium/well was transferred to another 96-well plate. The solution of LDH substrate (50 μl) was added to the medium and incubated for 30 min. Subsequently, 50 μl stop solution was added to stop the reaction and the absorbance was measured at 490 nm with an iMark Microplate Reader. The results were normalized to the control (the group treated with vehicle).

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Manufacturer protocol

Download the product protocol from Promega for CytoTox 96® Non-Radioactive Cytotoxicity Assay below.

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