NF-κB p65 (D14E12) XP® Rabbit mAb #8242

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Publication protocol

Formalin-fixed livers were embedded in paraffin, cut at 5 μm, and stained with hematoxylin and eosin or immunohistochemistry (IHC). Primary antibodies for IHC included rabbit antibodies against Ki-67-Ab 16667, DKK1-Ab 61034, INCENP-Ab36453 (Abcam, Cambridge, UK), cleaved caspase-3-9661, NF-κB-D14E12XP p65, c-Jun-9165 (Cell Signaling, Beverly, MA), β-catenin-RB9035 (ThermoFisher Scientific/Lab Vision, Fremont, CA). Heat-induced antigen retrieval was performed with citrate buffer, pH 6, for c-Jun, cleaved caspase-3, NF-κB p65 and β-catenin or with EDTA buffer, pH 8, for Ki-67, DKK1 and INCENP. Rabbit primary antibody binding was detected with goat anti-rabbit polymer HRP (ZytoChem Plus, Berlin, Germany). Color was developed with Diaminobenzidine substrate-chromogen (ThermoFisher Scientific/Lab Vision) and tissues were counterstained with hematoxylin.

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