Anti-UCP1 antibody (ab10983)

Protocol tips

Upstream tips	Protocol tips	Downstream tips

Publication protocol

Harvested samples of eWAT, iWAT and BAT were fixed in formalin and embedded in paraffin. Sections were stained using hematoxylin and eosin (H&E) and automated immunohistochemistry was performed (Ventana Medical Systems, Tucson, AZ) using anti-UCP1 and anti-myeloperoxidase (MPO) antibodies (Abcam, Cambridge, MA). Whole slide digital images were obtained from the resultant slides using an Aperio AT2 digital slide scanner (Leica Microsystems, Buffalo Grove, IL). The high resolution digital images were analyzed using Image Scope™ software (Aperio version 12) using the positive pixel count algorithm as described by Gannon et al. (21). In brief, the algorithm generates three classes of output values (weak positive, positive, and strong positive) based on pixel intensity which represents staining intensity. Only positive and strong positive values were included in the analysis. Three similar-sized areas were randomly selected on each digital slide and the average value of those three areas was used as the final value for comparison.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Immunohistochemistry Mouse - UCP1 using Anti-UCP1 antibody (ab10983) from Abcam.

Manufacturer protocol

Download the product protocol from Abcam for Anti-UCP1 antibody (ab10983) below.

Videos

Check out videos that might be relevant for performing Immunohistochemistry Mouse - UCP1 using Anti-UCP1 antibody (ab10983) from Abcam. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.