Anti-UCP-1 antibody produced in rabbit

Protocol tips

Upstream tips	Protocol tips	Downstream tips

Publication protocol

iWAT samples were embedded in paraffin and sectioned. After blocking with goat serum (rabbit IgG kit, Vector Laboratory, Burlingame, CA, USA) for 45 min, the sections were incubated with the primary antibody (rabbit anti-UCP1 antibody, Sigma u6382, Sigma-Aldrich, St. Louis, MO, USA) at a dilution of 1:1600 at 4°C. The following day, the sections were incubated with a secondary antibody (Rabbit IgG kit, Vector Laboratory, Burlingame, CA, USA), avidin-biotin - complex (ABC) solution, and then a diaminobenzidine solution (DAB peroxidase substrate kit, Vector Laboratory, Burlingame, CA, USA) at room temperature. Sections were washed in between each step with phosphate-buffered saline and Tween-20 (0.2%). iWAT images were captured using an Olympus BX41 light microscope.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Immunohistochemistry Mouse - UCP1 using Anti-UCP-1 antibody produced in rabbit from Sigma-Aldrich.

Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Anti-UCP-1 antibody produced in rabbit below.

Videos

Check out videos that might be relevant for performing Immunohistochemistry Mouse - UCP1 using Anti-UCP-1 antibody produced in rabbit from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.