Publication protocol
Hematoxylin and eosin staining was performed on fixed tissue sections using Harris Modified Hematoxylin and Eosin Y Solution (Sigma, St. Louis, MO), according to the manufacturer's instructions. Immunostaining was done using standard protocols [17]. Briefly, sections were fixed with 4% paraformaldehyde in PBS. Non-specific binding was blocked with 10% normal serum diluted in 1% bovine serum albumin (BSA, Jackson Lab, USA) and 0.25% Triton X-100 for one hour in room temperature. The sections were then incubated with primary antibodies diluted with 1% BSA + 0.25% Triton X-100 at 4°C overnight. The sections were then incubated with appropriate secondary antibodies (Cy3 or Cy2 conjugated antibodies (Jackson Lab) diluted with 1% BSA + 0.25% Triton X-100 or Alexa Fluor 488, Alexa Fluor 594, and Alexa 647 (1:1000, Invitrogen)) in the dark at room temperature for 2 hours. Counterstaining was then performed with DAPI (1:5000). Fluorescent images were processed by Adobe Photoshop. Anti-Ki67 (Novus Biologicals), anti-total β-cat (Santa Cruz Biotechnology Inc), anti-phospho β-cat (Thr41/Ser45) (cell signaling), anti-active β-cat (clone 8E7, Millipore), and NSE (BioGenex), anti-K14 (covance) are used in this study.
Full paper
Login or
join for free to view the full paper.
Manufacturer protocol
Download the product protocol from Biogenex for Anti-NSE, Clone MIG-N3 below.
We haven't found the manufacturer protocol for this product yet.
Videos
Check out videos that might be relevant for performing Immunohistochemistry Mouse - NSE using Anti-NSE, Clone MIG-N3 from Biogenex. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.
We haven't found any additional videos for this experiment / product combination yet.