Anti-EpCAM antibody (ab71916)

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Publication protocol

Heat-induced epitope retrieval was performed on 4-μm formalin-fixed, paraffin-embedded sections utilizing a pressurized Decloaking Chamber (Biocare Medical LLC, Concord, CA) in citrate buffer (pH 6.0) at 99°C for 18 minutes. Fluorescence: Slides were incubated in a normal serum and BSA blocking step at room temperature for 20 minutes. After incubation with primary antibody overnight at 4°C, slides were labeled with Alexa Fluor dye-conjugated secondary antibody and mounted with ProLong Gold (Invitrogen).

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