Cell Counting Kit-8

Protocol tips

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	- Before cells are treated with control medium and CCK8 reagent, cells are treated with increasing concentrations (25, 50, 75, and 100 nM) of oleandrin. cells were incubated at 37 °C for 3 h.

Protocol tips
- Before cells are treated with control medium and CCK8 reagent, cells are treated with increasing concentrations (25, 50, 75, and 100 nM) of oleandrin. cells were incubated at 37 °C for 3 h.

Publication protocol

U2OS or SaOS-2 cells were seeded in a 96-well dish at a final density of 5 × 103 (U2OS) or 1 × 104 (SaOS-2) cells/well and were incubated for 24 h. The cells were then treated with oleandrin at increasing concentrations (25, 50, 75, and 100 nM) and the control medium for 24, 48 and 72 h. Thereafter, 10 μl CCK-8 (Dojindo Laboratories, Kumamoto, Japan) was added to each well and incubated for another 3 h. The absorption at 450 nm was determined for each sample using an automatic ELISA plate reader. Five replicate wells were used for each treatment, and the experiments were repeated three times. Cell viability (%) = [OD (treatment)-OD (blank)]/[OD (control)-OD (blank)] × 100 %.

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Manufacturer protocol

Download the product protocol from Dojindo for Cell Counting Kit-8 below.

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