Anti-REG1 alpha antibody (ab231092)

Protocol tips

Upstream tips	Protocol tips	Downstream tips

Publication protocol

Pancreases (12, 36-week old) were perfused and fixed in 4% paraformaldehyde in 0.1 M PBS for 24 hours at 4°C, paraffin embedded and sectioned (4 μm). Paraffin-fixed tissue sections were blocked with 5% bovine serum albumin (BSA) for 30 min, incubated overnight at 4°C with rabbit anti-mouse Reg1 (1:200, Abcam, Cambridge, UK) or rabbit anti-mouse EXTL3 (1:200, Santacruz, Dallas, USA), then washed and incubated with HRP-linked anti-rabbit serum at room temperature for 30 min. The sections were then developed with DAB, counterstained with hematoxylin, and examined using an Olympus BX40 microscope.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Immunohistochemistry Mouse - Reg1 using Anti-REG1 alpha antibody (ab231092) from Abcam.

Manufacturer protocol

Download the product protocol from Abcam for Anti-REG1 alpha antibody (ab231092) below.

Videos

Check out videos that might be relevant for performing Immunohistochemistry Mouse - Reg1 using Anti-REG1 alpha antibody (ab231092) from Abcam. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.