Anti-REG1 alpha antibody (ab231092)

Immunohistochemistry Mouse - Reg1

Experiment
Immunohistochemistry Mouse - Reg1
Product
Anti-REG1 alpha antibody (ab231092) from Abcam
Manufacturer
Abcam

Protocol tips

Publication protocol

Pancreases (12, 36-week old) were perfused and fixed in 4% paraformaldehyde in 0.1 M PBS for 24 hours at 4°C, paraffin embedded and sectioned (4 μm). Paraffin-fixed tissue sections were blocked with 5% bovine serum albumin (BSA) for 30 min, incubated overnight at 4°C with rabbit anti-mouse Reg1 (1:200, Abcam, Cambridge, UK) or rabbit anti-mouse EXTL3 (1:200, Santacruz, Dallas, USA), then washed and incubated with HRP-linked anti-rabbit serum at room temperature for 30 min. The sections were then developed with DAB, counterstained with hematoxylin, and examined using an Olympus BX40 microscope.

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Papers

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Manufacturer protocol

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