Mouse Reg1 Antibody

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Publication protocol

Paraffin-embedded sections were dehydrated with increasing concentrations of ethanol (80–100%). After three 15-min washings with PBS, samples were blocked for 20 min in 3% normal donkey serum at 37°C and then incubated overnight at 4°C with guinea pig anti-murine insulin (A0564; Dako/Agilent Technologies, Carpinteria, CA) and sheep anti-murine Reg1 primary antibodies. Following three more washes with PBS, the sections were incubated with AffiniPure donkey anti-guinea pig Alexa Fluor 488 and anti-sheep Rhodamine Red-X (706-545-148 and 713-295-147, respectively; Jackson ImmunoResearch) secondary antibodies for 30 min at 37°C. The samples were then washed three times with PBS, mounted with SlowFade Diamond Antifade medium containing DAPI (Invitrogen), and visualized by confocal microscopy.

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