Ad-Sal-shRNA

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	The diabetic rats were re-divided into three groups, which respectively received intravenous injection of PBS (DM group), adenoviral vectors encoding scramble shRNA (2.0 × 1010 plaque-forming units, DM/Ad-Scr-shRNA group) or adenoviral vectors encoding salusin-β shRNA (2.0 × 1010 plaque-forming units, DM/Ad-Salusin-shRNA group). The intravenous injections were repeated 2 weeks after the first administration.14, 38 The rats were killed for measurements 4 weeks after the first time of intravenous injection (20 weeks after feeding with HFD). Ad-Salusin-shRNA and Ad-Scr-shRNA were constructed by Genomeditech Co. (Shanghai, China), which downregulated the salusin-β expression by 75%. The efficiency of Ad-Sal-shRNA in knockdown of salusin-β was confirmed in rats in our previous study.

Protocol tips

The diabetic rats were re-divided into three groups, which respectively received intravenous injection of PBS (DM group), adenoviral vectors encoding scramble shRNA (2.0 × 1010 plaque-forming units, DM/Ad-Scr-shRNA group) or adenoviral vectors encoding salusin-β shRNA (2.0 × 1010 plaque-forming units, DM/Ad-Salusin-shRNA group). The intravenous injections were repeated 2 weeks after the first administration.14, 38 The rats were killed for measurements 4 weeks after the first time of intravenous injection (20 weeks after feeding with HFD). Ad-Salusin-shRNA and Ad-Scr-shRNA were constructed by Genomeditech Co. (Shanghai, China), which downregulated the salusin-β expression by 75%. The efficiency of Ad-Sal-shRNA in knockdown of salusin-β was confirmed in rats in our previous study.

Publication protocol

The diabetic rats were re-divided into three groups, which respectively received intravenous injection of PBS (DM group), adenoviral vectors encoding scramble shRNA (2.0 × 1010 plaque-forming units, DM/Ad-Scr-shRNA group) or adenoviral vectors encoding salusin-β shRNA (2.0 × 1010 plaque-forming units, DM/Ad-Salusin-shRNA group). The intravenous injections were repeated 2 weeks after the first administration.14, 38 The rats were killed for measurements 4 weeks after the first time of intravenous injection (20 weeks after feeding with HFD). Ad-Salusin-shRNA and Ad-Scr-shRNA were constructed by Genomeditech Co. (Shanghai, China), which downregulated the salusin-β expression by 75%. The efficiency of Ad-Sal-shRNA in knockdown of salusin-β was confirmed in rats in our previous study.

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