RIPA Buffer

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Cells were washed twice with ice cold PBS and lysed in RIPA buffer containing protease and phosphatase inhibitor cocktails (Sigma). Protein content was measured with BCA reagent (Pierce).

Protocol tips
Cells were washed twice with ice cold PBS and lysed in RIPA buffer containing protease and phosphatase inhibitor cocktails (Sigma). Protein content was measured with BCA reagent (Pierce).

Publication protocol

Cells were washed twice with ice cold PBS and lysed in RIPA buffer containing protease and phosphatase inhibitor cocktails (Sigma). Protein content was measured with BCA reagent (Pierce).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein isolation Mammalian cells - Human lung fibroblasts using RIPA Buffer from Sigma-Aldrich.

Manufacturer protocol

Download the product protocol from Sigma-Aldrich for RIPA Buffer below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Protein isolation Mammalian cells - Human lung fibroblasts using RIPA Buffer from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.