MMP-3 (D7F5B) Rabbit mAb #14351

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The animal protocol was approved by the Ethics and Animal Research Committee of Huazhong University of Science and Technology. We purchased male Sprague-Dawley rats weighing 250-300g from the Experimental Animal Center of Tongji Medical College (Wuhan, China) and induced OA as described previously [37]. The animals (n=5/group) were randomly divided into blank control, OA, and experimental groups. OA pathogenesis was induced by surgery involving anterior cruciate ligament transaction and partial medial meniscectomy [37]. The experimental group rats were fed with 25 mg/ Kg/ day tomatidine as previously described [38]. After 12 weeks, the animals were sacrificed, the tibiofemoral joints were collected, fixed with 4% paraformaldehyde for 24h, and decalcified by incubation in 10% EDTA for 20 days. Then, the specimens were embedded in wax blocks, cut into 3-5μm thick sections, and stained with safranin O-fast green(S-O) and toluidine blue. The stained specimens from all 3 rat groups were scored according to the Osteoarthritis Research Society International (OARSI) recommendations and graded in a blinded manner. Another set of tibiofemoral joint sections from the 3 groups were incubated with antibodies against INOS and MMP3. The numbers of INOS and MMP3 positive cells were determined using the Image-J software.

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