SECISBP2 Polyclonal antibody

Protocol tips

Upstream tips	Protocol tips	Downstream tips

Publication protocol

DA rats cartilage tissues were fixed in 4% buffered paraformaldehyde for more than 2 days and subsequently decalcified with EDTA (12.5% EDTA, pH 7.4). Cartilage tissue sections were embedded in paraffin and 4 μm-thick sections were cut. Immunohistochemistry staining was performed using the standard streptavidin peroxidase complex method. Firstly, tissue sections were deparaffinized in xylene and rehydrated through a graded series of alcohols. Next, tissue sections were subjected to H2O2 treatment and antigen retrieval, and subsequently blocked with 5% bovine serum albumin (BSA) for 30 min at room temperature. Tissue sections were incubated with primary antibodies at 4°C overnight. After sequential incubations with biotinylated secondary antibody (BOSTER, catalog SA1022, China) and horseradish peroxidase-conjugated avidin (BOSTER, catalog SA1022, China), protein staining was performed using the Diaminobenzidine Substrate Kit (BOSTER, catalog SA2022, China). Samples were counterstained with hematoxylin, and the resulting brown staining indicated the proteins immunoreactivity. The results of IHC were observed under microscope with 400× magnification and quantification was performed with the Image-Pro® Plus software. The antibody information is depicted in Table S3.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Immunohistochemistry Rat - SBP2 using SECISBP2 Polyclonal antibody from Proteintech Group.

Manufacturer protocol

Download the product protocol from Proteintech Group for SECISBP2 Polyclonal antibody below.

Videos

Check out videos that might be relevant for performing Immunohistochemistry Rat - SBP2 using SECISBP2 Polyclonal antibody from Proteintech Group. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.