Anti-GFAP antibody (ab7260)

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Publication protocol

Spinal cord sections were subjected to xylene dewaxing, gradient alcohol rehydration, and antigen retrieval with boiling 0.01 M citrate buffer. Endogenous peroxidase activity was eliminated by 15 min of incubation with 3% hydrogen peroxide. Then, these sections were incubated with goat serum blocking solution for 15 min at room temperature and incubated with primary antibodies (rabbit anti-rat NF200, ab8135, GFAP, ab7260, GAP-43, ab75810, 1:100, Abcam, UK) for 12 h at 4°C. After washing 3 times with PBS, sections were incubated with horseradish peroxidase-labeled goat anti-rabbit IgG (1:500, Beijing Zhongshang Jinqiao Biotechnology Co., Ltd.) for 15 min at 37°C. Followed by DAB color reaction and hematoxylin counterstaining, sections were sealed with neutral gum and observed under an optical microscope (Olympus). Positive cells were counted in 5 random fields of view.

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