CyQUANT® Cell Proliferation Assay Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- 5000 cells were seeded and incubated at 37 °C in a 5% CO2 atmosphere for 10 days	- The cell culture medium was refreshed every two days

Upstream tips
- 5000 cells were seeded and incubated at 37 °C in a 5% CO2 atmosphere for 10 days
Protocol tips
- The cell culture medium was refreshed every two days

Publication protocol

RGD-modified and uncoated Mg AZ31 samples were sterilized as described above and placed in the wells of a 24-well plate. Five thousand cells (in 600 μL McCoy's medium) were seeded on the substrates and incubated at 37 °C in a 5% CO2 atmosphere for 10 days. The cell culture medium was refreshed every two days. After the required incubation period, the number of cells on the substrates was measured using the CyQUANT assay kit as described above.

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