Recombinant Anti-MUC1 antibody [EPR1023] (ab109185)

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Paraffin-embedded, formalin-fixed LMS and LPS specimens were used for IHC. Sections were incubated overnight in a humidified container at 4°C with the primary antibodies of MUC1 (1:100, ab109185; Abcam) and CD11c (1:100, ab52632, Abcam). After three times washing, tissue sections were incubated with the secondary antibody conjugated with streptavidin–horseradish peroxidases for 1 h at room temperature. The slides were stained with 3, 3-diaminobenzidine tetrahydrochloride (DAB) and the nuclei were counterstained with hematoxylin. Immunostaining on each slide was assessed by experienced pathologists to examine the percentage of MUC1 or CD11c positive tumor cells and presented as histochemistry score (H-score). H-score = Σpi(i+1) where i is the intensity score and pi is the percent of the cells with that intensity.

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