MUC6 Polyclonal Antibody

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"A tissue microarray technique was used for immunohistochemical evaluation as described previously [18]. Briefly, tissue microarrays (TMAs) were constructed using a manual tissue-arraying instrument (Beecher, Silver Spring, MD, USA). Between 3 and 14 (mean 5.03, median 5) cylindrical core biopsies, 0.6 mm in diameter, were taken from different sites of each tumor and arrayed in a recipient paraffin TMA.

For immunohistichemical staining, 4 μm TMA sections were treated with 1 % H2O2, subjected to antigen retrieval and subsequently incubated for 30 min with primary antibodies using automated staining systems (Universal Staining System, DakoCytomation, Glostrup, Denmark, or BenchMark, Ventana Medical Systems, S.A, Illkirch CEDEX, France). Details on primary antibodies, dilution, and epitope retrieval are listed in Table ​Table1.1. The reaction was visualized by the Dako EnVision system detection kit, or the ultraVIEW Universal DAB detection kit (Ventana). Goblet cells in adjacent non-neoplastic colonic mucosa served as positive control for MUC2, slides of gastric cancer, known to express MUC1, MUC5AC, and MUC6 served as positive control for MUC1, MUC5AC, and MUC6. Negative controls included omission of the primary antibodies and incubation with Dako ChemMate Antibody Diluent (code no. S 2022) or Ventana Antibody Diluent (catalog no. 251–018), respectively."

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