CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- After 24hrs of cell seeding, FC101 (0–5 µM) was added. Each well was added 20 µl of one solution reagent and incubated for 1 h.	- Optical density (OD) is measured at 490 nm.

Protocol tips
- After 24hrs of cell seeding, FC101 (0–5 µM) was added. Each well was added 20 µl of one solution reagent and incubated for 1 h.
Downstream tips
- Optical density (OD) is measured at 490 nm.

Publication protocol

Cell proliferation was also evaluated by one solution assay, using CellTiter 96 AQueous One Solution Cell Proliferation Assay kit (Promega), according the manufacturer’s instructions. Briefly, cells suspended in the growth medium were seeded in a 96-well plate at a density of 1×104 cells per well (in triplicates). The next day, FC101 (0–5 µM) was added. After incubation for 24 h, each well was added 20 µl of one solution reagent and incubated for 1 h. Cell proliferation was determined by measuring the optical density (OD) at 490 nm using a Wallac 1420 Multilabel Counter (PerkinElmer Life Sciences, Wellesley, MA). Cells treated with vehicle alone (PBS) served as a control

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Manufacturer protocol

Download the product protocol from Promega for CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) below.

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