pANC210 (xylB)

Protein Expression Prokaryotic cells - A. cellulolyticus xylB

Experiment
Protein Expression Prokaryotic cells - A. cellulolyticus xylB
Product
pANC210 (xylB) from Kazuhiko Ishikawa, Biomass Refinery Research Center, National In
Manufacturer
Kazuhiko Ishikawa, Biomass Refinery Research Center, National In

Protocol tips

Protocol tips
Protoplasts of A. cellulolyticus YP-4 were transformed with pANC209, 210, 223, 230, 231, 232 and 233 by nonhomologous integration into the host chromosomal DNA (Fujii et al. [2012]). Gene integration into prototrophic transformants was verified by genomic PCR. All cultures were grown in medium containing 20 g/l soluble starch and 5 g/l urea using the method described previously (Inoue et al. [2013]). The recombinant xylanases expressed were purified from the culture supernatant.

Publication protocol

Protoplasts of A. cellulolyticus YP-4 were transformed with pANC209, 210, 223, 230, 231, 232 and 233 by nonhomologous integration into the host chromosomal DNA (Fujii et al. [2012]). Gene integration into prototrophic transformants was verified by genomic PCR. The expression of each recombinant xylanase was carried out using the following cultures: Y209 (YP-4 transformed with pANC209; XylA), Y210 (YP-4 transformed with pANC210; XylB), Y223 (YP-4 transformed with pANC223; XylC), Y230 (YP-4 transformed with pANC230; XylD), Y231 (YP-4 transformed with pANC231; XylE), Y232 (YP-4 transformed with pANC232; XylF), Y233 (YP-4 transformed with pANC233; XylG) (Table 2). All cultures were grown in medium containing 20 g/l soluble starch and 5 g/l urea using the method described previously (Inoue et al. [2013]). The recombinant xylanases expressed were purified from the culture supernatant.

Full paper   Login or join for free to view the full paper.

Reviews

pANC210 (xylB) from Kazuhiko Ishikawa, Biomass Refinery Research Center, National In has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Protein Expression Prokaryotic cells - A. cellulolyticus xylB using pANC210 (xylB) from Kazuhiko Ishikawa, Biomass Refinery Research Center, National In.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Kazuhiko Ishikawa, Biomass Refinery Research Center, National In for pANC210 (xylB) below.

We haven't found the manufacturer protocol for this product yet.

Videos

Check out videos that might be relevant for performing Protein Expression Prokaryotic cells - A. cellulolyticus xylB using pANC210 (xylB) from Kazuhiko Ishikawa, Biomass Refinery Research Center, National In. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms